Applying antibiotic selection markers for nematode genetics.
نویسندگان
چکیده
Antibiotic selection markers have been recently developed in the multicellular model organism Caenorhabditis elegans and other related nematode species, opening great opportunities in the field of nematode transgenesis. Here we describe how these antibiotic selection systems can be easily combined with many well-established genetic approaches to study gene function, improving time- and cost-effectiveness of the nematode genetic toolbox.
منابع مشابه
Concerns of resistant markers in marine ecosystem transformed plants
World population growth and requirement to global food security, application of genetic engineering and utilization of transgenic organisms have made more important. Using this technology, without regarding to its risks, can cause loses to environment. To generate transgenic organisms, selection systems are applied that cause to selective growth of transformed cells. Antibiotic resistance genes...
متن کاملGreen Nanoparticles Engineering on Root-knot Nematode Infecting Eggplant plants and Their Effect on Plant DNA Modification
Background: Root-knot nematodes are known to cause significant damage to eggplants. New approaches by green silver nanoparticles (GSN) are used to control plant-parasitic nematode to avoid chemical nematicide hazards.Objectives: Analyses of the incorporation of different concentrations of nanoparticles on two different algae (Ulva lactuca and Turbinaria turbinata) were carried out. Fureth...
متن کاملRelationship between Psidium species (Myrtaceae) by resistance gene analog markers: focus on nematode resistance.
Guava (Psidium guajava L.) crop is severely affected by the nematode Meloidogyne enterolobii. Native Psidium species have been reported as sources of resistance against this nematode. Knowledge on the molecular relationship between Psidium species based on plant resistance gene analogs (RGA) can be useful in the genetic breeding of guava for resistance to M. enterolobii. In this study, RGA mark...
متن کاملMicrosatellites reveal genetic diversity in Rotylenchulus reniformis populations.
Rotylenchulus reniformis is the predominant parasitic nematode of cotton in the Mid South area of the United States. Although variable levels of infection and morphological differences have been reported for this nematode, genetic variability has been more elusive. We developed microsatellite-enriched libraries for R. reniformis, produced 1152 clones, assembled 694 contigs, detected 783 simple ...
متن کاملMarker assisted selection for the improvement of Sarjoo-52 for drought tolerance by introgression of MQTL1.1 from the source Nagina–22
Literatures have reported that a lot of drought related genes were cloned and individual gene showed positive effects under controlled stress experiments, but were not much effective in the field. Although, the progresses by conventional breeding approaches were achievable as some drought varieties have been released to the farmers in the recent years but this is not adequate to cope up with th...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Methods
دوره 68 3 شماره
صفحات -
تاریخ انتشار 2014